Linie hrachu AGT 210.31

Dostálová Radmila, Ing.

hrách, výsledky (výzkumu a vývoje)
Line pea AGT210.31 was created during the research project MSM 2678424601. Line has orange cotyledons - gene Orc. AGT 210.31 was developed from cross Bilbo x 4876. The selection of individual plants was repeated every year from the F2 generation. Yield tests were performed since the F4 generation, the 3 x 10 m2 yield trials. Breeding line AGT 210.31 was included in yield trials in 3 trial locations maturity and was sown (sowing density: 1.0 mil of germinating seeds per hectare) with standard varieties. The homogenization process usually takes 3-4 years, morphologically different and less efficient lines were excluded during the process. funkční vzorek

Morpho-colorimetric traits of Pisum seeds measured by an image analysis system

Smýkalová Iva, Ing., PhD., Bjelková Marie, Ing., Ph.D., Hýbl Miroslav, Ing. Ph.D.

Grillo, O.; Venora, G.hrách, výsledky (výzkumu a vývoje)
Pisum sativum L., as well as many other grain legumes has high agronomical importance connected to their use in crop rotations. Color, size and shape of seeds are very important in grading system for many crops. A total of 33 morphometric and colorimetric features of seeds of five Czech pea varieties, cultivated in four different Czech localities, were measured by image analysis and used to identify and discriminate the varieties also regarding to their cropping localities. The achieved data were used to develop a statistical classifier based on two sample sets, the training set to teach the classifier and the test sets to validate it, and determine the percentage of varietal identification. The developed image analysis system for pea seeds was able to highlight : 1) differences among varieties, 2) the effect of locality on pea seeds traits, 3) different stability of variety. článek v odborném periodiku

Molekulární detekce genu rezistence hrachu k viru mozaiky přenosné semenem (PSbMV)

Smýkal Petr, Ing., Ph.D.

hrách, výsledky (výzkumu a vývoje)
Pea seed-borne mosaic virus (PSbMV) belongs among the most frequent viral pathogens causing severe losses in field pea and other legumes. These losses might be prevented by growth of resistant varieties. Reliable markers of viral resistances were not available until now. Recently eIF4E resp. eIF4E(iso) genes were identified, corresponding to sbm-1 (LG VI) and sbm-2 (LG II) loci, governing recessive resistance to potyviruses, including PSbMV. We identified alelles of eIF4E gene in selected pea genotypes, including all used resistance donors. Based on sequences we have selected and verified PCR-based markers, allowing 100% precise identification, including distinction of heterozygotes. Thus this method in contrast to tedious virological and imunological tests offers faster, cheaper and precise results directly usable in pea breeding. certifikovaná metodika

Linie hrachu AGT 210.10

Dostálová Radmila, Ing.

hrách, výsledky (výzkumu a vývoje)
Line pea AGT 205.21 was created during the research project MSM 2678424601. Line has very good resistanceto complex diseases in field conditions. AGT 210.10 was developed from cross (US 1533 x AO. 904) x 4876. The selection of individual plants was repeated every year from the F2 generation. Yield tests were performed since the F4 generation, the 3 x 10 m2 yield trials. Breeding line AGT 210.10 was included in yield trials in 3 trial locations maturity and was sown (sowing density: 1,0 mil of germinating seeds per hectare) with standard varieties. The homogenization process usually takes 3-4 years, morphologically different and less efficient lines were excluded during the process. funkční vzorek

Metodika pěstování sóji luštinaté

Hýbl Miroslav, Ing. Ph.D., Ondřej Michal, RNDr. CSc., Bubeník Josef, Ing., Ponížil Antonín, Ing., Mgr., CSc.

M. Houba; J. Holečeksoja, výsledky (výzkumu a vývoje)
The publication aims to highlight the possible risks in growing soybeans and to point out the numerous advantages and benefits. The purpose of the system is to provide information about significant changes in the properties of varieties and their cultivation technology important points to ensure a positive impact on the economic viability of farms. certifikovaná metodika

Metodika ochrany žita proti námelovitosti

Ondráčková Eliška, Mgr., Ondřej Michal, RNDr. CSc.

Cagaš, Bohumírochrana rostlin, výsledky (výzkumu a vývoje)
This technologies describe the methods how to reduce the ergot incidence in the rye growth. It is particularly intended for growers, seed growers and breeders of rye, triticosecale and grasses. Recommended methodologies arose from a study of the causes and effects which cause a higher incidence of ergot sclerotia in the rye growth. certifikovaná metodika

Metodika agrobakteriální genetické transformace lnu (Linum usiatissimum L.)

Prokopová Miroslava, Ing., Ph.D.

len, výsledky (výzkumu a vývoje)
A routine procedure for most efficient genotypes independent genetic transformation of flax, and possibly other industrial crops. The procedure is a guideline for the preparation of sterile seeds, preparation of media for in vitro culture and genetic transformation of their own. certifikovaná metodika

Listová skvrnitost bobu

Ondřej Michal, RNDr. CSc., Ondráčková Eliška, Mgr.

bob; vikev, výsledky (výzkumu a vývoje)
In field trials the susceptibility of colour- and white-flowering faba bean varieties against cercospora leaf spot (Cercospora zonata) was determined. In green-mature stage the white-flowering varieties Albi and Merlin were attacked at scores 4.2 and 3.6, respectively. The colour-flowering varieties Merkur and AGT 5/13 were attacked at scores 7.0 and 7.2, respectively. At harvest time the attachment of white-flowering varieties Merlin and Albi with diseases complex was 85.5 % and 60 % respectively in comparison with attachment 30 % and 16 % of colour-flowering varieties Merkur and AGT 5/13, respectively. The viability of Cercospora zonata conidia is low (3–5 days) and the fungus is soil borne. Dormant structures of fungus surviving in the soil are the primary source of crop infestation. článek v odborném periodiku

Metodika pěstování lupiny bílé, žluté a úzkolisté

Hýbl Miroslav, Ing. Ph.D., Ondřej Michal, RNDr. CSc., Seidenglanz Marek, Ing., Ph.D., Vaculík Antonín, Ing., Ph.D.

lupina, metodika, výsledky (výzkumu a vývoje)
The purpose of the article is to provide growers and processors of information on the biological characteristics of the resulting demands on the growing conditions and cultivation practices lupine white, yellow and leaved as an important source of crude protein in the diet of farm animals, an alternative to soy šrotům extracted. For completeness and lupine reported variable, the possibility of its use and method of cultivation. The aim of the methodology is to show possibilities of lupine and comprehensively characterize critical agronomic measures affecting the quality and quantity achieved production. certifikovaná metodika

AGT 578/03 – genetický zdroj velmi nízkého obsahu antinutričních látek v semeni lnu

Tejklová Eva, RNDr., Bjelková Marie, Ing., Ph.D.

výsledky (výzkumu a vývoje)
AGT 578/03 is characterized by low content of antinutritional substances (cyanogenic glycosides) in the seed compared with registered varieties of linseed in the Czech Republic. funkční vzorek

Accumulation of cadmium by flax and linseed cultivars in field-simulated conditions: A potential for phytoremediation of Cd - contaminated soils

Bjelková Marie, Ing., Ph.D., Griga Miroslav, RNDr., CSc.

Václava Genčurovávýsledky (výzkumu a vývoje)
The possibility of use of two technological types of Linum usitatissimum L., namely flax (grown for fibre) and linseed (grown for seed), for phytoextraction of cadmium (Cd) from Cd-contaminated soil was studied. A four-year field-simulated experiment was carried out with 6 flax and 4 linseed cultivars in order to study organ accumulation of Cd by flax and linseed plants at artificial concentration range 10 to 1000 mg Cd . kg-1 soil. The most Cd was accumulated by roots, followed by shoots, while reproductive parts (capsules and seeds) played comparably smaller role. The increasing soil Cd concentration resulted in increasing Cd accumulation by roots, while transport to above-ground plant parts was progresivelly inhibited. Even high soil Cd concentrations (1000 mg Cd . kg-1 soil) had not dramatic negative effect on plant growth and development.differences as well as the differences between both technological Linum types have been found in Cd accumulation (flax being better Cd accumulator than linseed). Nevertheless, the recorded variation between technological types and within is in multiples of Cd values (units of mg Cd . kg-1 DW), not in as needed for practical phytoextraction. A significant year-to-year effect on plant growth/development resulting in high variation in Cd accumulation was observed. Further, the contrasting in total Cd accumulation (high accumulating flax cv. Jitka versus low accumulating linseed cv. Jupiter) were selected for future experiments. The uptake of Cd by flax/ linseed from ha per season was calculated and the strategy for flax/linseed growing on heavy metal polluted soils with subsequent utilization of heavy metal-contaminated biomass is discussed. článek v odborném periodiku

Bílá hniloba řepky Sclerotinia sclerotiorum – její výskyt a význam na Opavsku a Šumpersku

Poslušná Jana, Ing.

Plachká, Eva
Sclerotinia stem rot, caused by polyphagous pathogen Sclerotinia sclerotiorum, is significant disease of winter oilseed rape in Czech Republic and also all over the world. The Sclerotinia ascospores presence was monitored on the rape buds and floral leaves in 2007 – 2010 at 2 chosen localities (Opava, Šumperk). The source of infection is the ascospores of the patogen. The patogen was detected and cultivated from collected floral leaves on the cultivating medium (PDA). The supposed damage caused by patogen was defined on the base on percentage of infected petals (collected in growth stage BBCH 61-65). The stubble disease occurrence evaluation was done before the harvest of oilseed rape and the degree of damage was recorded. The gained results were statistically compared. The level of infection depends on the measure of the occurrence of the infection source and weather conditions at a given fields. článek v odborném periodiku

Kompatibilní směs dvou půdních mykoparazitických hub umožňující výrobu biopreparátu

Ondřej Michal, RNDr. CSc.

výsledky (výzkumu a vývoje)
This is a unique mixture of two mycoparasitic soil fungi Clonostachys rosea (SCL-01 strain) and Trichoderma asperellum-harzianum (ASPH strain). The mixture of both fungi generates a culture named GXT-08, which is secure stored in Czech collection of microorganisms. Both components of mixture can be easily cultivated on both liquid and solid substrates, and they are suitable for production of bio-product. Both submerge and surface methods can be used in manufacturing of bio-product. užitný vzor

AGT 181/08 - genetický zdroj vysokého obsahu tuku v semeni a vysokého obsahu kyseliny linolenové v tuku

Tejklová Eva, RNDr.

Rakouský Slavomír
Linseed line AGT 181/08 with a very high fat content in the seeds and very high alpha-linolenic acid content in seed fat was obtained by regeneration from in vitro cultured hypocotyl segment of a linseed variety Biltstar with blue flowers and brown seeds. AGT 181/08 owercomes the original variety Biltstar and variety Flanders, the only one variety of linseed of the same type registered in the Czech Republic, in fat content in seeds and in omega-3 fatty acid - linolenic acid - content. AGT 181/08 bears breeding valuable markers - white star-shaped flowers and yellow-gray color of the seeds. None of linseed varieties registered in the State Variety Book has the same type of flower and seed color. It can be asserted as a source of genetic characteristics listed in linseed breeding for industrial use or for use the whole seeds in food production or as a source of omega-3 fatty acid in supplementary nutrition. ostatní